Minus and plus strand

ACTGGCGGCCATGGAACTCACCG

X - 15353623 15353645

SO this mapped to the minus strand

To match the plus strand, first reverse the sequence to:

GCCACTCAAGGTACCGGCGGTCA

Then compliment the reversed sequence to:

CGGTGAGTTCCATGGCCGCCAGT

Then it will be mapped to:

X + 15353623 15353645